Serum-Free Medium Evokes Cyclooxygenase-2 Pathway in A549 Cells
نویسندگان
چکیده
منابع مشابه
Serum-Free Medium Evokes Cyclooxygenase-2 Pathway in A549 Cells
Purpose: The main aim of the present study was to find out if serum-free treatment was able to up-regulate cyclooxygenase-2 (COX-2) pathway in A549 cells which were treated with serum-free medium for different hours. Methods: Prostaglandin E2 (PGE2, a prostaglandin mainly from COX-2 pathway) in the supernatant synthesized by A549 cells, were determined with PGE2 enzyme immunoassay kits. The pro...
متن کاملScreening method for nonsteroidal antiinflammatory drugs based on the cyclooxygenase 2 pathway activated by serum-free stimulation in A549 cells.
Cyclooxygenase 2 (COX-2) pathway inhibitors were regarded as promising nonsteroidal antiinflammatory drugs (NSAIDs). We discovered that the COX-2 pathway in A549 cells, a human lung cancer cell line, was activated by serum-free stimulation, and a drug screening model for NSAIDs was established based on this principle with simple performance and sufficient reliability. The COX-2 pathway was acti...
متن کاملEGF stimulates cyclooxygenase-2 expression through the STAT5 signaling pathway in human lung adenocarcinoma A549 cells.
The epidermal growth factor receptor (EGFR) can be activated by several growth factors within the tumor microenvironment, and it can activate several signaling pathways. For tumor development, these EGFR-related signaling pathways may converge on several common nuclear transcription factors, one such transcription factor being STAT5. STAT5 plays an important role in the oncogenic signal transdu...
متن کاملStructural Changes in Hela Cells Cultivated in Serum-free Medium
In general, it is still impossible, except in a few instances (7, 9), to cult ivate an imal and h u m a n cells successfully in serum-free medium. W h e n cul t ivated under such conditions, the cells proliferate for only a l imited period of t ime and then stop. I t seems reasonable to assume tha t differences in the consti tuents of the med ium which have such a marked influence on pat terns ...
متن کاملAdipocyte conversion of CHEF cells in serum-free medium
When grown in the presence of serum with added insulin, Chinese hamster embryonic fibroblasts (CHEF/18) cells can be induced to become preadipocytes that are committed to the adipocyte pathway of terminal differentiation (Sager, R., and P. Kovac, 1982, Proc. Natl. Acad. Sci. USA, 79:480-484). We found that commitment to the adipocyte pathway, as well as terminal differentiation to form mature a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Open Inflammation Journal
سال: 2009
ISSN: 1875-0419
DOI: 10.2174/1875041900902010034